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Sorbonne University is committed to the success of each of its students. Au cours de son cycle de vie L. L'objectif de ces travaux pratiques est de construire des mutants n'exprimant pas de flagelle DfliA, DflaA,..
Guennadi Sezonov Pr. The laboratory techniques and study methodology described provide accurate and useful information for the control of epidemics using a minimum of resources. Often, the countries that face the challenge of responding to an epidemic are those with the least resources.
Therefore, the microbiology laboratory must use its resources wisely in order to have the greatest impact on reducing morbidity and mortality during an epidemic. This manual is intended for use by Reference Laboratories. To receive email updates about this topic, enter your email address: Email Address. Picture of second MAC plate. Observe the colonies on the MacConkey Agar plate.
Do the colonies appear to be the same type observed previously? How do the foregoing procedures and observations fit in with Koch's Postulates? Assign each task you have done in this protocol to a postulate. Links 1. You have studied some of the physiology of bacteria [1] as they relate to culture media and biochemical testing. You probably know where in this manual you can find information on specific bacterial characteristics and the procedures for the various tests. The purpose of this exercise is to correlate the various bits of information to strengthen your understanding of, and familiarity with 1 the media and tests used this semester and 2 the major characteristics of the genera of bacteria with which you have had some experience.
We have been using typical species of these genera throughout the course. Dichotomous Key The exercise will consist of two parts. First, you will prepare a Figure [2] and Figure 2 [3]. Use tests such as the primary tests considering any of the secondary tests! Some things to consider in making your key: 1. If you believe that either a primary test e. The only instance where lactose fermentation was for us of realistic importance in differentiating between genera was in Experiment 14 for the enteric bacteria [5].
Can glucose fermentation be used to differentiate Bacillus from anything else such as Lactobacillus? Go back to Section [6] and note that we studied several species of Bacillus - some of which were facultative anaerobes [7]. That should answer the question. Where you may have had some experience with an organism having a variable reaction or a weakly or delayed positive reaction e. Use something more definitive! Where you may not know a reaction for a particular organism H2S for Bacillus?
Glucose fermentation for Neisseria? One does not have to know all of the reactions for the 12 organisms to make a dichotomous key. With these things in mind, you can prepare a key which will be of use in characterizing typical members of these genera, even outside of the course.
This key should be submitted no later than the two weeks before the course ends. It will then be graded and handed back in time for you to consider which tests you will need to identify your unknowns.
Isolation and Identification of Unknowns The second part of this endeavor involves the actual isolation and identification of the three components of the mixed unknown. One typical representative of each of three genera on the list below will be found in this mixture. Naturally you should want to start off by checking the mixture with a gram [8] stain and then streaking for isolated colonies [9].
Note that you have two media for this purpose. One - Heart Infusion Agar - is an all-purpose medium [10] which will support the growth of all 12 genera. The other - MacConkey Agar - is a selective- differential medium which will in this experiment support the growth of only the gram- negative rods and will differentiate between any two or more different organisms growing on it.
Which may be best to prevent "overgrowth? Remember to record your unknown number. Figure Possible organisms in the test Bacillus Staphylococcus Neisseria Micrococcus Morganella Enterobacter Pseudomonas Klebsiella Citrobacter Lactobacillus Escherichia Enterococcus The table shows the possible genera of the species of microbes that you may find in this test.
You must design a dichotomous key to help you work out the identity of your microbe. You may use any of the media or reagents listed here to identify your isolate. This is more than sufficient and please don't ask for media or reagents that are not on this list. Don't forget the Gram stain! How old should the culture be?
Can a reliable Gram stain be made directly from a MacConkey Agar colony? What about oval cells? Are they rods or cocci?
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